4.7 Article

Tnfaip2/exoc3-driven lipid metabolism is essential for stem cell differentiation and organ homeostasis

期刊

EMBO REPORTS
卷 22, 期 1, 页码 -

出版社

WILEY

关键词

Exoc3; lipid metabolism; organ homeostasis; stem cell differentiation; Tnfaip2

资金

  1. German Research Foundation (DFG) within the CRC-PolyTarget
  2. European Union (Advance ERC grant) [323136]
  3. European Research Council (ERC) [323136] Funding Source: European Research Council (ERC)

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The study identifies Tnfaip2 and Smed-exoc3 as key regulators of lipid metabolism, essential for ESC differentiation and planarian organ maintenance. Tnfaip2 knockout impairs ESC differentiation, while depletion of Smed-exoc3 affects tissue homeostasis and regeneration in planarians.
Lipid metabolism influences stem cell maintenance and differentiation but genetic factors that control these processes remain to be delineated. Here, we identify Tnfaip2 as an inhibitor of reprogramming of mouse fibroblasts into induced pluripotent stem cells. Tnfaip2 knockout impairs differentiation of embryonic stem cells (ESCs), and knockdown of the planarian para-ortholog, Smed-exoc3, abrogates in vivo tissue homeostasis and regeneration-processes that are driven by somatic stem cells. When stimulated to differentiate, Tnfaip2-deficient ESCs fail to induce synthesis of cellular triacylglycerol (TAG) and lipid droplets (LD) coinciding with reduced expression of vimentin (Vim)-a known inducer of LD formation. Smed-exoc3 depletion also causes a strong reduction of TAGs in planarians. The study shows that Tnfaip2 acts epistatically with and upstream of Vim in impairing cellular reprogramming. Supplementing palmitic acid (PA) and palmitoyl-L-carnitine (the mobilized form of PA) restores the differentiation capacity of Tnfaip2-deficient ESCs and organ maintenance in Smed-exoc3-depleted planarians. Together, these results identify a novel role of Tnfaip2 and exoc3 in controlling lipid metabolism, which is essential for ESC differentiation and planarian organ maintenance.

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