期刊
EXPERIMENTAL AND THERAPEUTIC MEDICINE
卷 21, 期 1, 页码 -出版社
SPANDIDOS PUBL LTD
DOI: 10.3892/etm.2020.9496
关键词
Salvia miltiorrhiza; salvianic acid A; salvianolic acid B; human ovarian granulosa tumor cells; oxidation
资金
- Research Foundation of Administration of Traditional Chinese Medicine of Hebei Province [2016111]
The study demonstrated the protective effect of Salvia miltiorrhiza solution and its active compounds on H2O2-induced cell damage in vitro, which was achieved by reducing oxidative stress and decreasing apoptosis in ovarian granulosa tumor cells.
The dried roots or rhizomes of Salvia miltiorrhiza Bge are commonly used in Chinese medicine to promote blood circulation and regulate menstruation. Salvianic acid A and salvianolic acid B are the main active water-soluble compounds in Salvia miltiorrhiza solution. The present study investigated the protective effect of Salvia miltiorrhiza solution and its active compounds in H2O2-induced cell damage of the human ovarian granulosa tumor cell line (KGN) in vitro, as well as its underlying mechanism. Cell viability was detected using a Cell Counting Kit-8 assay. In addition, the levels of malondialdehyde (MDA), superoxide dismutase (SOD), glutathione (GSH) and tumor necrosis factor-alpha (TNF-alpha) were measured. Western blotting was performed to detect the protein expression of cleaved caspase-3 and caspase-9. Furthermore, immunocytochemistry was used to detect the expression of TNF-alpha. It was demonstrated that Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B did not affect the viability of KGN cells. Additionally, salvianic acid A and salvianolic acid B significantly reduced the H2O2-induced increased MDA levels, and reversed the H2O2-induced suppression of SOD and GSH activities in KGN cells (P<0.05). Treatment with Salvia miltiorrhiza solution, salvianic acid A and salvianolic acid B significantly reduced the overexpression of cleaved caspase-3, cleaved caspase-9 and TNF-alpha compared with the H2O2-treated group (P<0.05). Therefore, the present results indicated that Salvia miltiorrhiza solution and its main water-soluble compounds, salvianic acid A and salvianolic acid B, ameliorated KGN cell damage induced by H2O2.
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