期刊
JOURNAL OF KOREAN NEUROSURGICAL SOCIETY
卷 64, 期 1, 页码 100-109出版社
KOREAN NEUROSURGICAL SOC
DOI: 10.3340/jkns.2020.0071
关键词
Diffuse astrocytic tumor; Histopathology grade; Mutant IDH1 R132H; Ki-67; PD-L1
资金
- Indonesian Ministry of Research and Technology (National Research & Innovation Agency) [16/E1/KPT/2020]
The study revealed a high frequency of IDH1 mutations in grade II DAT, with differences in Ki-67 and PD-L1 expression between different histopathological grades. An association was found between mutant IDH1 R132H and Ki-67 with PD-L1 expression in DAT.
Objective : Diffuse astrocytic tumour (DAT) is a diffuse infiltrative astrocytoma tumour accompanied by molecular parameters such as the presence or absence of isocitrate dehydrogenase (IDH) gene mutations. Ki-67 is a marker for DAT proliferation, while programmed death ligand 1 (PD-L1) indicates an immune evasion mechanism. This study aimed to analyze the correlation among mutant IDH1 R132H, Ki-67, and PD-L1 immunoexpression in the DAT. Methods : A cross-sectional study was carried out on 30 paraffin blocks of DAT cases. Paraffin block samples consist of grade II (n=14), grade III (n=8), and grade IV (n=8). In this study, the immunohistochemistry-staining of mutant IDH1 R132H, Ki-67, and PD-L1 were carried out to determine the frequency of DAT with IDH1 mutations. Results : Our study shown the frequency of IDH1 mutations in grade II 50.0% (7/14), grade III 37.5% (3/8), and grade IV 12.5% (1/8). Our study also showed a difference in Ki-67 and PD-L1 expression between each the degree of DAT histopathology (p=0.0001 and p=0.002, respectively). There was an association between both mutant IDH1 R132H, and Ki-67 with PD-L1 expression in DAT (p=0.0087 and p=0.0049, respectively). Conclusion : DAT with the mutant IDH1 is frequently observed in grade II and small number of grade III. The expression of wild type IDH1, Ki-67, and PD-L1 were found to be higher in high grade DAT (grade III and grade IV). There is a correlation between each of mutant IDH1 status and Ki-67 with PD- L1 expression in DAT.
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