Impact of elevated CO2 and heat stress on wheat pollen viability and grain production

Periods of high temperature and an expected increase in atmospheric CO2 concentration as a result of global climate change are major threats to wheat (Triticum aestivum L.) production. Developing heat-tolerant wheat cultivars demands improved understanding of the impacts of high temperature and elevated CO2 on plant growth and development. This research investigated the interactive effects of heat stress and CO2 concentration on pollen viability and its relationship to grain formation and yield of wheat in greenhouse conditions. Nineteen wheat genotypes and a current cultivar, Suntop, were heat stressed at either meiosis or anthesis at ambient (400 mu L L-1) or elevated (800 mu L L-1) CO2. Elevated CO2 and heat stress at meiosis reduced pollen viability, spikelet number and grain yield per spike; however, increased tillering at the elevated CO2 level helped to minimise yield loss. Both heat-tolerant genotypes (e.g. genotype 1, 2, 10 or 12) and heat-sensitive genotypes (e.g. genotype 6 or 9) were identified and response related to pollen sensitivity and subsequent impacts on grain yield and yield components were characterised. A high-throughput protocol for screening wheat for heat stress response at elevated CO2 was established and meiosis was the most sensitive stage, affecting pollen viability, grain formation and yield.

Automated summary

This research examined the interactive effects of heat stress and CO2 concentration on wheat pollen viability and its relationship to grain formation and yield, highlighting that heat-tolerant wheat genotypes can help minimize yield loss under elevated CO2 levels.