4.8 Article

Signal-enhanced real-time magnetic resonance of enzymatic reactions at millitesla fields

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CHEMICAL SCIENCE
卷 12, 期 1, 页码 314-319

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ROYAL SOC CHEMISTRY
DOI: 10.1039/d0sc04884d

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  1. Max Planck Society
  2. German Research Foundation (DFG) [418416679, 426677227]

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This study demonstrates the real-time monitoring of enzymatic reactions at lower magnetic fields through signal enhancement via hyperpolarization, paving the way for portable and affordable NMR systems in biological studies with potential for medical diagnostics.
The phenomenon of nuclear magnetic resonance (NMR) is widely applied in biomedical and biological science to study structures and dynamics of proteins and their reactions. Despite its impact, NMR is an inherently insensitive phenomenon and has driven the field to construct spectrometers with increasingly higher magnetic fields leading to more detection sensitivity. Here, we are demonstrating that enzymatic reactions can be followed in real-time at millitesla fields, three orders of magnitude lower than the field of state-of-the-art NMR spectrometers. This requires signal-enhancing samples via hyperpolarization. Within seconds, we have enhanced the signals of 2-C-13-pyruvate, an important metabolite to probe cancer metabolism, in 22 mM concentrations (up to 10.1% +/- 0.1% polarization) and show that such a large signal allows for the real-time detection of enzymatic conversion of pyruvate to lactate at 24 mT. This development paves the pathways for biological studies in portable and affordable NMR systems with a potential for medical diagnostics.

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