Structural dynamics of DNA depending on methylation pattern

DNA methylation is associated with a number of biological phenomena, and plays crucial roles in epigenetic regulation of eukaryotic gene expression. It is also suggested that DNA methylation alters the mechanical properties of DNA molecules, which is likely to affect epigenetic regulation. However, it has not been systematically investigated how methylation changes the structural and dynamic features of DNA. In this research, to elucidate the effects of methylation on DNA mechanics, a fully atomic molecular dynamics simulation of double-stranded DNA with several methylation patterns was performed. Through the analysis of the relative positioning of the nucleotides (base-step variables), characteristic changes in terms of local flexibility were observed, which further affected the overall DNA geometry and stiffness. These findings may serve as a basis for a discussion on methylation-dependent DNA dynamics in physiological conditions.

Automated summary

DNA methylation is associated with biological phenomena and epigenetic regulation, and can alter the mechanical properties of DNA molecules. This study used molecular dynamics simulations to show that methylation patterns affect the local flexibility of DNA, ultimately impacting its overall geometry and stiffness. These findings provide insights into methylation-dependent DNA dynamics in physiological conditions.