Microfluidic assay of antiplatelet agents for inhibition of shear-induced platelet adhesion and activation

We have developed a microfluidic system to perfuse whole blood through a flow channel with an upstream stenotic region and a downstream protein capture region. This flow-based system was used to assay how effectively antiplatelet agents suppress shear-induced platelet adhesion and activation downstream of the stenotic region. Microcontact printing was used to covalently attach one of three platelet binding proteins [fibrinogen, collagen, or von Willebrand factor (vWf)] to the surface of the downstream capture region. Whole blood with an antiplatelet agent was transiently exposed to an upstream high wall shear rate (either 4860 s(-1) or 11 560 s(-1)), and subsequently flowed over the downstream capture region where the platelet adhesion was measured. Several antiplatelet agents (acetylsalicylic acid, tirofiban, eptifibatide, anti-vWf, and anti-GPIb alpha) were evaluated for their efficacy in attenuating downstream adhesion. Following antibody blocking of vWf or GPIb alpha, downstream platelet activation was also assessed in perfused blood by flow cytometry using two activation markers (active GPIIb/IIIa and P-selectin). Acetylsalicylic acid demonstrated its inability to diminish shear-induced platelet adhesion to all three binding proteins. GPIIb/IIIa inhibitors (tirofiban and eptifibatide) significantly reduced platelet adhesion to fibrinogen. Antibody blocking of vWf or GPIb alpha effectively diminished platelet adhesion to all three capture proteins as well as platelet activation in perfused blood, indicating an essential role of vWf-GPIb alpha interaction in mediating shear-induced platelet aggregation.

Automated summary

A microfluidic system was developed to assess the effectiveness of antiplatelet agents in suppressing shear-induced platelet adhesion and activation. Results showed that certain antiplatelet agents significantly reduced downstream platelet adhesion, with GPIIb/IIIa inhibitors showing particularly strong effects. Antibody blocking of vWf or GPIb alpha effectively diminished platelet adhesion and activation, highlighting the importance of the vWf-GPIb alpha interaction in mediating shear-induced platelet aggregation.