期刊
INTERNATIONAL JOURNAL OF MEDICAL SCIENCES
卷 18, 期 5, 页码 1225-1239出版社
IVYSPRING INT PUBL
DOI: 10.7150/ijms.51743
关键词
Pineal gland; RNA sequencing; microRNAs; circular RNAs
资金
- National Natur al Science Foundation of China [31872349]
In this study, a large number of circRNAs and miRNAs were identified in the rat pineal gland through transcriptome analysis, with differential expression patterns during the day and night. A ceRNA regulatory network was constructed to predict interactions among circRNAs, miRNAs, and mRNAs with differential expression, providing insights into the role of circRNA in regulating changes in melatonin circadian rhythms. The analyzed data presented in this study will serve as a valuable resource for future investigations on the altered physiology of circRNAs in diurnal rhythms.
Circular RNAs (circRNAs) are a new class of covalently closed circular RNA molecules that are involved in many biological processes. However, information about circRNAs in the pineal gland, particularly that of rats, is limited. To establish resources for the study of the rat pineal gland, we performed transcriptome analysis of the pineal glands during the day and night. In this study, 1413 circRNAs and 1989 miRNAs were identified in the pineal gland of rats during the night and day using the Illumina platform. Forty differentially expressed circRNAs and 93 differentially expressed miRNAs were obtained, among which 20 circRNAs and 37 miRNAs were significantly upregulated during the day and 20 circRNAs and 56 miRNAs were significantly upregulated during the night. As circRNAs have been reported to work as miRNA sponges, we predicted 15940 interactions among 40 circRNAs, 93 miRNAs and 400 mRNAs with differential diurnal expression using miRanda and TargetScan to build a ceRNA regulatory network in the rat pineal gland. The diurnal expression profile of circRNAs in the rat pineal gland may provide additional information about the role of circRNAs in regulating changes in melatonin circadian rhythms. The analyzed data reported in this study will be an important resource for future studies to elucidate the altered physiology of circRNAs in diurnal rhythms.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据