期刊
SOFT MATTER
卷 17, 期 2, 页码 254-267出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0sm01097a
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资金
- H2020-NMBP-TR-IND2018
- EVPRO (Development of Extracellular Vesicles loaded hydrogel coatings with immunomodulatory activity for Promoted Regenerative Osseointegration of revision endoprosthesis) grant [814495-2]
- National Science Foundation [DMR-1807127, DMR1720530]
- P. Roy Vagelos Chair at Penn
- EU
- federal state of North Rhine-Westphalia [EFRE 30 00 883 02]
This study demonstrates how changes in amphiphile topology in the phospholipid bilayer can drive shape transformations of cell membrane models. By designing and synthesizing new types of amphiphiles-Janus dendrimers-with a photo-labile bond, shape changes are induced, allowing for control of budding and the generation of asymmetrical structures.
The vital functions of cell membranes require their ability to quickly change shape to perform complex tasks such as motion, division, endocytosis, and apoptosis. Membrane curvature in cells is modulated by very complex processes such as changes in lipid composition, the oligomerization of curvature-scaffolding proteins, and the reversible insertion of protein regions that act like wedges in the membrane. But, could much simpler mechanisms support membrane shape transformation? In this work, we demonstrate how the change of amphiphile topology in the bilayer can drive shape transformations of cell membrane models. To tackle this, we have designed and synthesized new types of amphiphiles-Janus dendrimers-that self-assemble into uni-, multilamellar, or smectic-ordered vesicles, named dendrimersomes. We synthesized Janus dendrimers containing a photo-labile bond that upon UV-Vis irradiation cleavage lose a part of the hydrophilic dendron. This leads to a change from a cylindrically to a wedge-shaped amphiphile. The high mobility of these dendrimers allows for the concentration of the wedge-shaped amphiphiles and the generation of transmembrane asymmetries. The concentration of the wedges and their rate of segregation allowed control of the budding and generation of structures such as tubules and high genus vesicles.
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