期刊
CELL REPORTS MEDICINE
卷 2, 期 2, 页码 -出版社
CELL PRESS
DOI: 10.1016/j.xcrm.2021.100204
关键词
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资金
- NIH NIAID [AI42742, 75N9301900065, 75N93019C00001]
- NIH NIAID (NIH) [U01 CA260541-01]
- UCSD [AI007036, AI007384]
- Jonathan and Mary Tu Foundation
- Clinical and Experimental Immunology Course at the University of Genoa, Italy
- NIH NIAID (K08 award) [AI135078, AI036214]
By studying T cell responses in 99 convalescent COVID-19 cases, we identified various HLA-restricted epitopes derived from SARS-CoV-2 and observed distinct patterns of immunodominance. The epitopes were combined into megapools to facilitate the identification and quantification of virus-specific CD4(+) and CD8(+) T cells.
T cells are involved in control of SARS-CoV-2 infection. To establish the patterns of immunodominance of different SARS-CoV-2 antigens and precisely measure virus-specific CD4(+) and CD8(+) T cells, we study epitope-specific T cell responses of 99 convalescent coronavirus disease 2019 (COVID-19) cases. The SARS-CoV-2 proteome is probed using 1,925 peptides spanning the entire genome, ensuring an unbiased coverage of human leukocyte antigen (HLA) alleles for class II responses. For HLA class I, we study an additional 5,600 predicted binding epitopes for 28 prominent HLA class I alleles, accounting for wide global coverage. We identify several hundred HLA-restricted SARS-CoV-2-derived epitopes. Distinct patterns of immunodominance are observed, which differ for CD4(+) T cells, CD8(+) T cells, and antibodies. The class I and class II epitopes are combined into epitope megapools to facilitate identification and quantification of SARS-CoV-2-specific CD4(+) and CD8(+) T cells.
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