期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 59, 期 2, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02504-20
关键词
SARS coronavirus 2; COVID-19; serology; neutralization; seroepidemiology; human; animal; SARS-CoV-2; antibody; canine; cat; hamster; surrogate virus neutralization
类别
资金
- Health and Medical Research Fund, Food and Health Bureau, The Government of Hong Kong Special Administration Region [COVID190126]
The surrogate virus neutralization test (sVNT) showed high sensitivity and specificity compared to the plaque reduction neutralization test (PRNT90) in human, canine, cat, and hamster sera. There was no cross-reactivity to other coronaviruses in both SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.
Surrogate neutralization assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) that can be done without biosafety level 3 containment and in multiple species are desirable. We evaluate a recently developed surrogate virus neutralization test (sVNT) in comparison to 90% plaque reduction neutralization tests (PRNT90) in human, canine, cat, and hamster sera. With PRNT90 as the reference, sVNT had sensitivity of 98.9% and specificity of 98.8%. Using a panel of immune sera corresponding to other coronaviruses, we confirm the lack of cross-reactivity to other coronaviruses in SARS-CoV-2 sVNT and PRNT90, except for cross-reactivity to SARS-CoV-1 in sVNT.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据