期刊
JOURNAL OF CLINICAL MICROBIOLOGY
卷 59, 期 2, 页码 -出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/JCM.02236-20
关键词
bacterial vaginosis; application of Al to diagnostic microbiology; automation in clinical microbiology
类别
资金
- National Natural Science Foundation of China [81671409]
- Beijing Municipal Administration of Hospitals Clinical Medicine Development of Special Funding [XMLX201605]
- National Natural Science foundation of China (NSFC) [61532001]
- Tsinghua Initiative Research Program [20151080475]
- Ant Financial and Nanjing Turing AI Institute
A convolutional neural network model was developed to automatically identify and classify bacterial vaginosis Nugent scores from microscope images, outperforming human health care practitioners in terms of accuracy and stability.
Bacterial vaginosis (BV) is caused by the excessive and imbalanced growth of bacteria in vagina, affecting 30 to 50% of women. Gram staining followed by Nugent scoring based on bacterial morphotypes under the microscope is considered the gold standard for BV diagnosis; this method is often labor-intensive and time-consuming, and results vary from person to person. We developed and optimized a convolutional neural network (CNN) model and evaluated its ability to automatically identify and classify three categories of Nugent scores from microscope images. The CNN model was first established with a panel of microscopic images with Nugent scores determined by experts. The model was trained by minimizing the cross-entropy loss function and optimized by using a momentum optimizer. The separate test sets of images collected from three hospitals were evaluated by the CNN model. The CNN model consisted of 25 convolutional layers, 2 pooling layers, and a fully connected layer. The model obtained 82.4% sensitivity and 96.6% specificity with the 5,815 validation images when altered vaginal flora and BV were considered the positive samples, which was better than the rates achieved by top-level technologists and obstetricians in China. The capability of our model for generalization was so strong that it exhibited 75.1% accuracy in three categories of Nugent scores on the independent test set of 1,082 images, which was 6.6% higher than the average of three technologists, who are hold bachelor's degrees in medicine and are qualified to make diagnostic decisions. When three technologists ran one specimen in triplicate, the precision of three categories of Nugent scores was 54.0%. One hundred three samples diagnosed by two technologists on different days showed a repeatability of 90.3%. The CNN model outperformed human health care practitioners in terms of accuracy and stability for three categories of Nugent score diagnosis. The deep learning model may offer translational applications in automating diagnosis of bacterial vaginosis with proper supporting hardware.
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