4.6 Article

Antifouling hydrogel-coated magnetic nanoparticles for selective isolation and recovery of circulating tumor cells

期刊

JOURNAL OF MATERIALS CHEMISTRY B
卷 9, 期 3, 页码 677-682

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d0tb02380a

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资金

  1. National Natural Science Foundation of China [21904135]
  2. Natural Science Foundation of Jiangsu Province [BK20180250]
  3. Natural Science Foundation of Jiangxi Province [20192ACB21033]
  4. CAS International Cooperation Key program [121E32KYSB20170025]
  5. CAS/SAFEA International Innovation Teams program

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By coating magnetic nanoparticles with a hydrogel layer, high-efficiency capture and recovery of CTCs were achieved with high specificity and stable cell recovery rate.
For reliable downstream molecular analysis, it is crucially important to recover circulating tumor cells (CTCs) from clinical blood samples with high purity and viability. Herein, magnetic nanoparticles coated with an antifouling hydrogel layer based on the polymerization method were developed to realize cell-friendly and efficient CTC capture and recovery. Particularly, the hydrogel layer was fabricated by zwitterionic sulfobetaine methacrylate (SBMA) and methacrylic acid (MAA) cross-linked with N,N-bis(acryloyl)cystamine (BACy), which could not only resist nonspecific adhesion but also gently recover the captured cells by glutathione (GSH) responsiveness. Moreover, the anti-epithelial cell adhesion molecule (anti-EpCAM) antibody was modified onto the surface of the hydrogel to provide high specificity for CTC capture. As a result, 96% of target cells were captured in the mimic clinical blood samples with 5-100 CTCs per mL in 25 min of incubation time. After the GSH treatment, about 96% of the obtained cells were recovered with good viability. Notably, the hydrogel-coated magnetic nanoparticles were also usefully applied to isolate CTCs from the blood samples of cancer patients. The favorable results indicate that the hydrogel-modified magnetic nanoparticles may have a promising opportunity to capture and recover CTCs for subsequent research.

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