期刊
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
卷 9, 期 1, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.jece.2020.104925
关键词
E. coli; Impedimetric sensing; Differential pulse voltammetry; MIL-53; PEDOT:PSS; Composite
资金
- Department of Biotechnology (DBT), Ministry of Science and Technology, Government of India
- Department of Science and Technology (DST, India) [GAP-397 (EMR/2016/006480)]
This study developed an electrochemical biosensing system for E. coli detection, using a composite-modified electrode for specific detection within a wide concentration range. The results were validated against standard techniques, demonstrating successful detection and tolerance to possible interfering species.
Present work details the development of an electrochemical biosensing system for E. coli. A composite of a metal organic framework (MOF), i.e. MIL-53 (Fe) (MIL = Materials of Institut Lavoisier) with poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) has been prepared and used to modify the carbon-screen printed electrodes (cSPE). Due to the addition of PEDOT:PSS, the composite has exhibited electrochemical conductivity along with several other known beneficial features of MOF. The composite modified cSPE has been functionalized with anti-E. coli antibodies to guarantee the specificity of the detection. The present electrochemical sensor, based on the measurement of electrochemical impedance spectroscopic and different pulse voltammetry signals, has allowed the detection of E. coli over a wide concentration range of 2.1 x 10(2) to 2.1 x 10(8) cfu/mL, with the best detection limit of 4 cfu/mL. The sensor could also tolerate the co-presence of other possibly interfering species, such as S. aureus and S. arlettae. The results obtained from the present system have been validated against standard techniques.
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