期刊
CHEMICAL COMMUNICATIONS
卷 57, 期 11, 页码 1344-1347出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc07042d
关键词
-
By creating a chimera of Broccoli RNAs with mixed chemistries, it is possible to develop an effective RNA-based fluorescent K+ sensor that maintains structure and fluorescence properties without interference, providing linear fluorescent gain across physiological K+ concentrations.
The RNA aptamer Broccoli accepts 2 ' fluorinated (2 ' F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+ >30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+ concentrations, yielding an effective RNA-based fluorescent K+ sensor.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据