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Complete Genome Sequences of 12 Quinolone-Resistant Escherichia coli Strains Containing qnrS1 Based on Hybrid Assemblies

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AMER SOC MICROBIOLOGY
DOI: 10.1128/MRA.01190-20

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  1. European Union [773830]

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Using long-read sequencing on a MinION device and analysis with the MOB-suite pipeline, the genome sequences of 12 quinolone-resistant Escherichia coli strains containing qnrS1 were successfully assembled, with six of them closed after hybrid assembly.
In total, 12 quinolone-resistant Escherichia coli (QREC) strains containing qnrS1 were submitted to long-read sequencing using a FLO-MIN106 flow cell on a MinION device. The long reads were assembled with short reads (Illumina) and analyzed using the MOB-suite pipeline. Six of these QREC genome sequences were closed after hybrid assembly.

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