期刊
FUTURE MICROBIOLOGY
卷 12, 期 16, 页码 1457-1466出版社
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2017-0105
关键词
alpha-cyano-4-hydroxycinnamic acid matrix; acetonitrile; database; formic acid; log score; main spectrum profiles; MALDI-TOF MS; molds; MSPs; sabouraud dextrose agar; trifluoroacetic acid
类别
资金
- Department of Science and Technology, Government of India
Aim: To standardize the matrix-assisted laser desorption ionization-time of flight mass spectrometry protocols and expansion of existing Bruker Biotyper database for mold identification. Materials & methods: Four different sample preparation methods (protocol A, B, C and D) were evaluated. Results: On analyzing each protein extraction method, reliable identification and best log scores were achieved through protocol D. The same protocol was used to identify 153 clinical isolates. Of these 153, 123 (80.3%) were accurately identified by using existing database and remaining 30 (19.7%) were not identified due to unavailability in database. On inclusion of missing main spectrum profile in existing database, all 153 isolates were identified. Conclusion: Matrix-assisted laser desorption ionization-time of flight mass spectrometry can be used for routine identification of clinically important molds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据