Aggregation-induced emission enhancement of gold nanoclusters in metal-organic frameworks for highly sensitive fluorescent detection of bilirubin

A fluorescence analysis method based on gold nanocluster (AuNC) and metal-organic framework (MOF) composite materials (AuNCs@ZIF-8) was established for highly sensitive detection of bilirubin (BR). First, AuNCs@ZIF-8 was successfully obtained by co-precipitation and displayed an aggregation-induced emission enhancement by the confinement effect of the MOFs (i.e., ZIF-8). The product showed approximately 7.0 times enhancement in the quantum yield and longer fluorescence lifetime from 2.29 mu s to 11.51 mu s compared with AuNCs. When BR combined with the metal node Zn2+ of ZIF-8, the skeleton of the composite was destroyed, leading to a great decrease in the fluorescence intensity by the transformation of the AuNCs from the aggregated state to dispersed state. The linear range for the detection of BR was 0.1-5.0 mu M, with the limit of detection (LOD) of 0.07 mu M (S/N = 3). The AuNCs@ZIF-8 exhibited a selective response toward BR within 5 min and detected BR in human serum. The long-wavelength emission by AuNCs avoided the interference of the complex biomatrix background fluorescence, indicating their great application prospects for clinical diagnosis.

Automated summary

A fluorescence analysis method based on gold nanocluster and metal-organic framework composite materials was established for highly sensitive detection of bilirubin. The composite material displayed enhanced fluorescence properties, with a significant improvement in quantum yield and fluorescence lifetime compared to AuNCs alone. The method showed selective response and high sensitivity, making it suitable for clinical diagnosis.