A label-free electrochemical aptasensor based on the core-shell Cu-MOF@TpBD hybrid nanoarchitecture for the sensitive detection of PDGF-BB

As one of the significant serum cytokines, platelet-derived growth factor-BB (PDGF-BB) is a crucial protein biomarker overexpressed in human life-threatening tumors, the sensitive identification and quantification of which are urgently desired but challenging. Herein we report a novel core-shell nanoarchitecture consisting of Cu-based metal-organic frameworks (Cu-MOFs) and covalent organic frameworks (denoted as TpBD-COFs), which was used to prepare an aptasensor for the detection of platelet-derived growth factor-BB (PDGF-BB). The central Cu-MOFs function as signal labels with no need for extra redox media, whereas the porous TpBD serves as the shell to immobilize the PDGF-BB-targeted aptamer strands in abundance via strong interactions involving pi-pi stacking, electrostatic, and hydrogen bonding interactions. The proposed aptasensor based on Cu-MOF@TpBD can achieve a detection limit as low as 0.034 pg mL(-1) within the dynamic detection range from 0.0001 to 60 ng mL(-1). The hybridization of MOFs and COFs, together with the immobilization with the specific analyte targeted aptamer, provides a promising and propagable approach to prepare an aptasensor for the simple, sensitive, and selective detection of a specific biomarker in clinical diagnosis.

Automated summary

The novel core-shell nanoarchitecture consisting of Cu-based metal-organic frameworks (Cu-MOFs) and covalent organic frameworks (TpBD-COFs) can be used to prepare an aptasensor for the detection of platelet-derived growth factor-BB (PDGF-BB), achieving sensitive and selective detection of specific biomarkers.