4.7 Article

A ratiometric fluorescent probe for monitoring pH fluctuations during autophagy in living cells

期刊

CHEMICAL COMMUNICATIONS
卷 57, 期 12, 页码 1510-1513

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ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc07788g

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资金

  1. National Natural Science Foundation of China [21807029]
  2. Foundation of Henan Educational Committee [20A150036]
  3. Hubei Provincial Department of Education Science and Technology Research Projects [Q20182704]

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The ratiometric fluorescent probe presented in this study offers excellent photostability and chemostability for monitoring pH changes in living cells. Quantifying the down-regulation of intracellular pH during autophagy induced by various stimuli can provide valuable guidance for constructing autophagy models to study related pathological processes.
We present a ratiometric fluorescent probe for monitoring pH featuring superb photostability and chemostability. The down-regulation of the intracellular pH during autophagy in living cells induced by various stimuli, including several drugs and starvation, was quantified, which could provide instructive value to construct autophagy models to study the related pathological processes.

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