期刊
RSC ADVANCES
卷 11, 期 10, 页码 5491-5505出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra00343c
关键词
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资金
- Key Program of Natural Science Foundation of State [81830110, 81861168037, 81973745]
- Natural Science Foundation of Heilongjiang Province [YQ2019H030]
- Foundation of Heilongjiang University of Chinese Medicine [2018jc01, 201808]
- Heilongjiang Touyan Innovation Team Program
This study utilized high-throughput lipidomics combined with multivariate statistical analysis to identify 30 lipid biomarkers and 7 metabolic pathways associated with DR. The findings suggest that KLX intervention can regulate 22 biomarkers and the PI3K/MAPK pathway may be closely related to DR development. High-throughput lipidomics combined with multivariate statistical analysis can provide valuable insights into the molecular mechanisms and potential treatment targets for DR.
Diabetic retinopathy (DR) is an important complication of diabetes, and is currently the main cause of blindness among young adults in the world. Previous studies have shown that Keluoxin (KLX) capsules have a significant effect on DR in C57BL/KsJ/db(-/-) mice (db/db mice), however the unclear mechanism limits its further clinical application and actual value. Further research is urgently needed for the treatment of DR disease. Discovery of key lipid biomarkers and metabolic pathways can reveal and explore the molecular mechanisms related to DR development and discover the effect of Keluoxin (KLX) capsule against DR in db/db mice. Lipidomics has been used for characterizing the pathological conditions via identification of key lipid metabolites and the metabolic pathway. In this study, the high-throughput lipidomics using UHPLC-Q-TOF/MS combined with multivariate statistical analysis, querying multiple network databases and employing ingenuity pathway analysis (IPA) method for molecular target prediction. A total of 30 lipid biomarkers were identified and 7 metabolic pathways including arachidonic acid metabolism and steroid hormone biosynthesis were found. The preventive effect of KLX intervention can regulate 22 biomarkers such as LysoPA(16:0/0:0), prostaglandin D-2, cortisol and gamma-linolenic acid, etc. IPA platform has predicted that PI3K/MAPK pathway are closely related to DR development. It also showed that high-throughput lipidomics combined with multivariate statistical analysis could deep excavate of the biological significance of the big data, and can provide molecular targets information about the disease treatment.
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