Assisted breeding technology in the saltwater crocodile Crocodylus porosus: a review and look to the future

This review reports the current status of artificial breeding technology in the Crocodylia and the future requirements for the establishment of AI in the saltwater crocodile. Although there are challenges regarding safe restraint and immobilisation, semen collection of the saltwater crocodile by manual stimulation has proven effective in yielding sufficient volume and sperm concentrations for empirical and molecular analyses of sperm preservation and physiology. Nevertheless, there is still much to learn with respect to fundamental anatomy, physiology and behaviour in both sexes, but particularly in the female. Although lessons can be learned from successful AI in the alligator, the details of this research are not readily accessible. Future research needs to focus on the proximate factors of seasonality and the underlying control of the female's annual reproductive cycle; this will require novel and innovative ways to collect blood samples without causing stress or injury, and ideally a dedicated crocodile research breeding colony. Because the saltwater crocodile is a farmed species, there is likely to be sufficient impetus for the application of assisted breeding technology to drive future productivity in the industry. These developments will also have benefits for the genetic and reproductive management of endangered captive populations.

Automated summary

The review discusses the current status of artificial breeding technology in Crocodylia and the future requirements for establishing AI in the saltwater crocodile. It highlights the need for further understanding of anatomy, physiology, and behavior in crocodiles, especially females, as well as the importance of focusing on seasonality and the control of the female's reproductive cycle in future research. The application of assisted breeding technology in the saltwater crocodile industry is likely to drive future productivity and benefit the genetic and reproductive management of endangered captive populations.