期刊
HELIYON
卷 7, 期 1, 页码 -出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2020.e05673
关键词
Environmental science; Biological sciences; Microbiology; Biotechnology; Enzymology; Biobleaching; Co-culturing; Mannanase; Optimization; Solid-state fermentation; Xylanase
The study demonstrates that co-culturing strains in solid-state fermentation can significantly increase the yield of xylanase and mannanase, providing a promising alternative for reducing chemical use in the paper industry. The enzyme cocktail produced shows great potential for pulp biobleaching, resulting in a substantial reduction of kappa number and improved brightness and whiteness of the paper.
The Application of a combination of enzymes is the best alternative to reduce the use of chemicals in the paper industry. Bacillus sp. NG-27 and Bacillus nealsonii PN-11 are known to produce thermoalkali stable xylanse (X) and mannanase (M) respectively having potential for pulp biobleaching. The Present study, reports the production of a mixture of X + M by co-culturing of strains in SSF and standardizing its application for pulp biobleaching. Production of enzymes by co-cultivation in SSF was optimized by statistical methods. Substantial increase in the yield of enzymes; 3.61 fold of xylanase and 37.71 fold of mannanase was achieved. Application of enzyme cocktail for pulp biobleaching resulted in a 45.64% reduction of kappa number with 55 IU g(-1)odp of enzyme dose (xylanase:mannanase; 3:1) at pH 8.0 in 1h at 65 degrees C along with significant increase in brightness (11%) and whiteness (75%). The Same quality of paper as made up from chemical treated pulp can be made from enzyme-treated pulp with 30% less use of chlorine. Structural analysis of enzyme-treated pulp showed dissolution of hemicellulose as indicated by pores, cracks and increased roughness all over the surface. Cocktail of X + M produced economically in a single fermentation having all the requisite characteristics for pulp biobleaching is a highly suitable candidate for application in the pulp and paper industry.
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