期刊
JOURNAL OF BIOTECHNOLOGY
卷 328, 期 -, 页码 12-22出版社
ELSEVIER
DOI: 10.1016/j.jbiotec.2020.11.023
关键词
Photosynthesis; Algae; Cell growth; Photoprotection; NPQ; Biomass
资金
- University-Industry Joint Project Hypercell (grant JPVR 2016)
- University-Industry Joint Project Industrialgae (grant JPVR 2016)
- ENAC-2019 fund Alternative fuels for civil aviation
The study revealed that reducing photoprotective energy dissipation in Chlamydomonas algae does not effectively increase biomass productivity in photo-bioreactors. One strain lacking LHCSR1 and knocked down in LHCSR3 showed higher oxygen production under high light, along with enhanced PSII photodamage. Contrary to previous proposals, domestication of Chlamydomonas for carbon assimilation rate in photo-bioreactors by down-regulation of photoprotective energy dissipation was ineffective in increasing algal biomass productivity.
Unicellular green algae, a promising source for renewable biofuels, produce lipid-rich biomass from light and CO2. Productivity in photo-bioreactors is affected by inhomogeneous light distribution from high cell pigment causing heat dissipation of light energy absorbed in excess and shading of the deep layers. Contrasting reports have been published on the relation between photoprotective energy dissipation and productivity. Here, we have re-investigated the relation between energy quenching (qE) activity, photodamage and light use efficiency by comparing WT and two Chlamydomonas reinhardtii strains differing for their complement in LHCSR proteins, which catalyse dissipation of excitation energy in excess (qE). Strains were analysed for ROS production, protein composition, rate of photodamage and productivity assessed under wide light and CO2 conditions. The strain lacking LHCSR1 and knocked down in LHCSR3, thus depleted in qE, produced O-2 at significantly higher rate under high light, accompanied by enhanced singlet oxygen release and PSII photodamage. However, biomass productivity of WT was delayed in respect for mutant strains under intermittent light conditions only, implying that PSII activity was not the limiting factor under excess light. Contrary to previous proposals, domestication of Chlamydomonas for carbon assimilation rate in photo-bioreactors by down-regulation of photoprotective energy dissipation was ineffective in increasing algal biomass productivity.
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