Two Complete Mitochondrial Genomes From Leuctridae (Plecoptera: Nemouroidea): Implications for the Phylogenetic Relationships Among Stoneflies

The family-level relationships within Plecoptera have been a focused area of research for a long time. Its higher classification remains unstable, and the phylogenetic relationships within Plecoptera should be re-examined. Here, we sequenced and analyzed two complete mitochondrial genomes (mitogenomes) of Paraleuctra cercia and Perlomyia isobeae of the family Leuctridae. We reconstructed the phylogeny of Plecoptera based on 13 proteincoding genes (PCGs) from published stoneflies. Our results showed that the Bayesian inference and maximumlikelihood tree had similar topological structures except for the positions of two families, Peltoperlidae and Scopuridae.The Plecoptera is divided into two clades, the suborder Antarctoperlaria and the suborder Arctoperlaria. The two suborders subsequently formed two groups, Eusthenioidea and Gripopterygoidea, and Euholognatha and Systellognatha, which is consistent with the results of morphological studies. In addition, the Leuctridae is the earliest branch within the superfamily Nemouroidea. But the monophyly of Perloidea and Pteronarcyoidea are still not well supported.

Automated summary

Family-level relationships within Plecoptera have been a focused area of research, and the phylogenetic relationships within Plecoptera need to be re-examined. By sequencing and analyzing two complete mitogenomes from the family Leuctridae, it was revealed that Plecoptera is divided into two clades, which further formed four groups.