期刊
FUNGAL BIOLOGY
卷 121, 期 2, 页码 137-144出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.funbio.2016.11.003
关键词
Aroma; Endophyte; Limonene-1,2-diol; Submerged fermentation; Terpenoids
类别
资金
- Fundacao Araucaria [15637, 490/2010]
- CAPES scholarship
Aroma and fragrances have high commercial value for use in food, cosmetics and perfumes. The biotransformation of terpenes by microorganisms represents an attractive alternative method for production of flavourings. Endophytic fungi offer a great potential for the production of several groups of compounds; however, few studies have evaluated the biotransformation of limonene. Following preliminary studies on the biotransformation of limonene, submerged fermentation was carried out using an endophytic fungus isolated from Pinus taeda and identified as Phomopsis sp. The presence of several biotransformation products was detected and identified by mass spectrometry (GC-MS). The studied strain showed a divergent metabolic behaviour, as compounds of interest such as a-terpineol, carvone, and limoneno-1,2-diol were produced under different conditions. In addition to the minor metabolites terpinen-4- ol, menthol and carveol, this strain also produced major metabolites, including 0.536 g L-1 carvone and 2.08 g L-1 limonene-1,2-diol in synthetic medium and 2.10 g L-1 limonene-1,2-diol in a natural orange extract medium with single fed-batch, while the cyclic fed-batch resulted in concentrations less than 1 g L-1. Therefore, our study produced a wide variety of limonene derivatives at a high concentration using a natural medium and a newly isolated endophytic fungal strain. (C) 2016 British Mycological Society. Published by Elsevier Ltd. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据