期刊
PHARMACEUTICAL BIOLOGY
卷 59, 期 1, 页码 262-274出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/13880209.2021.1876100
关键词
Rheumatoid arthritis; osteoclast; RANKL; osteoprotegerin
资金
- National Science and Technology Major Project of the Ministry of Science and Technology of China [2018ZX09721004-009-002]
- Sichuan Science and Technology Program [2019JDRC0074]
- Xinglin Scholar Discipline Promotion Talent Program of Chengdu University of Traditional Chinese Medicine [BSH2018006]
Guizhi-Shaoyao-Zhimu decoction has shown anti-rheumatoid arthritis effects in CII-induced arthritis rats, suggesting its potential use as a supplement and alternative drug therapy for RA.
Context Guizhi-Shaoyao-Zhimu decoction (GSZD) is commonly used to treat rheumatoid arthritis (RA), but its mechanism is unclear. Objective To investigate the effect of GSZD on bone erosion in type II collagen (CII)-induced arthritis (CIA) in rats and to identify the underlying mechanism. Materials and methods The CIA model was prepared in male Wistar rats by two subcutaneous injections of CII, 1 mg/mL. Fifty CIA rats were randomized equally into the control group given saline daily, the positive group given saline daily and methotrexate 0.75 mg/kg once a week, and three GSZD-treated groups gavaged daily with 800, 1600 and 3200 mg/kg of GSZD for 21 days. GSZD effects were assessed by paw volume, arthritic severity index and histopathology. Cytokine levels were determined by ELISA. The effects of GSZD on RAW264.7 cells were evaluated by receptor activator of NF-kappa B ligand (RANKL)-induced osteoclastogenesis and bone resorption assay. Expression of I kappa B-alpha and p65 was measured by Western blotting. Major components of GSZD were identified by HPLC. Results Arthritis index score, paw volume and bone destruction score showed that GSZD improved inflammatory symptoms and reduced joint tissue erosion (p < 0.01). GSZD decreased RANKL, and the number of osteoclasts (OCs) in joint tissues (p < 0.01) and increased osteoprotegerin levels (p < 0.01). GSZD inhibited RANKL-induced RAW264.7 differentiation and reduced bone resorption by OCs. GSZD upregulated I kappa B (p < 0.01) and p65 (p < 0.01) in the cytoplasm and downregulated p65 (p < 0.01) in the cell nucleus. Conclusions Guizhi-Shaoyao-Zhimu decoction has an anti-RA effect, suggesting its possible use as a supplement and alternative drug therapy for RA.
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