期刊
FUNCTIONAL PLANT BIOLOGY
卷 44, 期 7, 页码 705-719出版社
CSIRO PUBLISHING
DOI: 10.1071/FP16424
关键词
carbohydrate; carbon allocation; carbon partitioning; salinity; salt stress; starch metabolism
资金
- Henry A Jastro Graduate Research Award
- Chinese Scholarship Council Scholarship from the Government of China
- France Berkeley Fund
- Hatch Project [CA-D-PLS-2164-H]
- Royal Thai Government
Rice (Oryza sativa L.) is very sensitive to soil salinity. To identify endogenous mechanisms that may help rice to better survive salt stress, we studied a rice GSK3-like isoform (OsGSK5), an orthologue of a Medicago GSK3 previously shown to enhance salinity tolerance in Arabidopsis by altering carbohydrate metabolism. We wanted to determine whether OsGSK5 functions similarly in rice. OsGSK5 was cloned and sequence, expression, evolutionary and functional analyses were conducted. OsGSK5 was expressed highest in rice seedling roots and was both salt and sugar starvation inducible in this tissue. A short-term salt-shock (150 mM) activated OsGSK5, whereas moderate (50 mM) salinity over the same period repressed the transcript. OsGSK5 response to salinity was due to an ionic effect since it was unaffected by polyethylene glycol. We engineered a rice line with 3.5-fold higher OsGSK5 transcript, which better tolerated cultivation on saline soils (EC = 8 and 10 dSm(-2)). This line produced more panicles and leaves, and a higher shoot biomass under high salt stress than the control genotypes. Whole-plant C-14-tracing and correlative analysis of OsGSK5 transcript with eco-physiological assessments pointed to the accelerated allocation of carbon to the root and its deposition as starch, as part of the tolerance mechanism.
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