期刊
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
卷 20, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1533033821997822
关键词
nasopharyngeal carcinoma; hsa-miR-9-5p; hexokinase 2; radiation
类别
This study demonstrated that hsa-miR-9-5p improved the radiosensitivity of nasopharyngeal carcinoma by targeting HK2. It led to decreased cell proliferation, increased apoptosis, and inhibited tumor growth in vivo after radiation therapy.
Background: This study was designed to explore the effects of hsa-miR-9-5p on radiotherapy sensitivity of nasopharyngeal carcinoma (NPC) by targeting hexokinase 2 (HK2). Methods: The levels of hsa-miR-9-5 and HK2 in NPC patients and radiosensitive and resistant cells were determined using qRT-PCR. The dual luciferase reporter gene system was used to determine hsa-miR-9-5p targeting HK2. The level of HK2 expression in NPC were determined using qRT-PCR and western blotting after the administration of hsa-miR-9-5p agomir. The effects of hsa-miR-9-5p on proliferation and apoptosis with or without irradiation (IR) were examined using CCK-8, flow cytometry and colony formation assays. (F-18)-Flourodeoxyglucose uptake was used to evaluate the growth of tumor with or without radiation therapy in vivo. Results: hsa-miR-9-5p target to inhibit HK2. Moreover, the cell proliferation was seen in a decreased trend while the cell apoptosis increased in the hsa-miR-9-5p group following radiation therapy hsa-miR-9-5p also showed a significant inhibitory effect on the growth of tumor in vivo with radiation therapy. Conclusions: hsa-miR-9-5p improved the radiosensitivity of NPC by targeting HK2.
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