Aptamer-mediated rolling circle amplification for label-free and sensitive detection of histone acetyltransferase activity

We develop for the first time an aptamer-mediated rolling circle amplification approach for label-free and sensitive detection of histone-modifying enzyme (HME) activity. This method can achieve femtomolar sensitivity for histone acetyltransferase Tip60 assay, which is the most sensitive HME assay reported so far. It can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.

Automated summary

We have developed an aptamer-mediated rolling circle amplification approach for the label-free and sensitive detection of histone-modifying enzyme activity. This method can achieve femtomolar sensitivity for the assay of histone acetyltransferase Tip60, and can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.