Protocol for isolation of spermatids from mouse testes

Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a sim-ple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).

Automated summary

Isolation of spermatid fractions using unit gravity sedimentation in a BSA density gradient can be used to analyze changes in transcript or protein expression during spermiogenesis.