期刊
STAR PROTOCOLS
卷 2, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2021.100365
关键词
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资金
- ERC Advanced Grant [833247]
- Netherlands Organization for Scientific Research
- CVON of Dutch Heart Foundation [CVON2018-27]
- Dutch Heart Foundation [667837]
- European Union Horizon 2020 Research And Innovation Program [ANR-16-CE15-0014-01]
- Netherlands Organization for Scientific Research [847507, 09150161910024]
- ANR JCJC [09150161810007]
- Institut Carnot-Microbes et Sante
- EU H2020 [11 CARN-017-01]
- Dutch Heart Foundation
- [2018T028]
- Agence Nationale de la Recherche (ANR) [ANR-16-CE15-0014] Funding Source: Agence Nationale de la Recherche (ANR)
The article outlines a protocol to recapitulate trained immunity in vitro using adherent monocytes isolated from peripheral blood mononuclear cells, and describes in detail the induction of trained immunity using b-glucan and Bacillus Calmette-Guerin as examples.
A growing number of studies show that innate immune cells can undergo functional reprogramming, facilitating a faster and enhanced response to heter-ologous secondary stimuli. This concept has been termed trained immunity.We outline here a protocol to recapitulate this in vitro using adherent monocytes from consecutive isolation of peripheral blood mononuclear cells. The induction of trained immunity and the associated functional reprogramming of monocytes is described in detail using b-glucan (from Candida albicans) and Bacillus Calmette-Guerin as examples. For complete details on the use and execution of this protocol, please refer to Repnik et al. (2003) and Bekkering et al. (2016).
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