Protocol Calcium imaging of adult-born neurons in freely moving mice

Adult-born neurons (ABNs) in the dentate gyrus bestow unique cellular plasticity to the mammalian brain. We recently found that the activity of ABNs during sleep is necessary for memory consolidation. Here, we describe our method for Ca2+ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings. As preparatory surgery and post-recording data processing can be major obstacles, we provide detailed descriptions and problem-solving tips. For complete details on the use and execution of this protocol, please refer to Kumar et al. (2020).

Automated summary

Adult-born neurons in the dentate gyrus play a crucial role in memory consolidation, particularly during sleep. This study presents a method for Ca2+ imaging of ABN activity using a miniaturized fluorescent microscope and sleep recordings, with detailed descriptions and tips provided for surgery and data processing challenges. For a comprehensive guide on the protocol, readers are encouraged to refer to Kumar et al. (2020).