期刊
STAR PROTOCOLS
卷 2, 期 1, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.xpro.2020.100275
关键词
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资金
- National Key Research and Development Program of China [2017YFA0106300]
- National Natural Science Foundation of China [81874226, 81803020, 91942309, 92057210]
- Thousand Talents Program for Distinguished Young Scholars
- International Scientific and Technological Cooperation Program from Guangdong Science and Technology Department [2018A050506033]
- Fundamental Research Funds for the Central Universities of China
- Three million for Three Years Project of the High-level Talent Special Funding Scheme of Sun Yat-sen Memorial Hospital
- Guangzhou Science and Technology Bureau [201902020015]
This protocol describes the design and preparation of an NP platform for mitochondria-specific gene delivery, which can be used in mice to deliver nucleic acid therapeutics and treat mitochondria-regulated diseases.
Targeted regulation of mitochondrial gene expression is challenging due to the lack of a mitochondria-specific delivery system. We have previously developed various stimuli-responsive nanoparticle (NP)-based delivery systems to transport nucleic acids for regulation of target gene expression. This protocol describes the design and preparation of an NP platform for mitochondria-specific gene de-livery (mito-NP). We use mito-NP in primary liver fibroblasts that are transplanted into mice. Mito-NP can be used to deliver various nucleic acid therapeutics and to treat mitochondria-regulated diseases.For complete details on the use and execution of this protocol, please refer to Zhao et al. (2020).
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