期刊
BIOMATERIALS SCIENCE
卷 9, 期 9, 页码 3433-3444出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0bm02201b
关键词
-
资金
- Jiangsu Key Research and Development Plan (Society Development) [BE2018639]
- Science AMP
- Technology Support Program of Changzhou [CJ20190033]
- International Scientific Cooperation Project of Changzhou Scientific Bureau [CZ20200015]
- QingLan Project of Jiangsu Province
- Natural Science Foundation of Jiangsu Province [BK20190097]
- Program of Qilu Young Scholars of Shandong University
- Taishan Scholars Program for Young Expert of Shandong Province [tsqn201909021]
- University Grants Committee of Hong Kong [N_CUHK422/18]
- CUHK RSFS grant
A gelatinase-responsive antibacterial photodynamic nanocomposite was designed to selectively kill S. aureus and promote rapid healing of infected wounds, showing significant photodynamic antibacterial activity in a mouse model of staphylococcal skin wound infection.
Staphylococcus aureus (S. aureus) related staphylococcal infection is one of the most common types of hospital-acquired infections, which requires selective and effective treatment in clinical practice. Considering gelatinase as a characteristic feature of S. aureus, gelatinase-responsive release of the antibiotic reagent thereby can target the pathogenic S. aureus while sparing beneficial bacteria in the microflora. In this work, we design a hybrid antibacterial photodynamic peptide (APP, Ce6-GKRWWKWWRRPLGVRGC) based on the polycationic antimicrobial peptide GKRWWKWWRR by introducing a photosensitizer chlorin e6 (Ce6) at the N-terminus, a cysteine residue at the C-terminus, and a gelatinase cleavage site (PLGVRG) inserted between the C-terminal cysteine and the polycationic peptide. This multi-motif peptide assembles with gold nanoclusters (AuNc) via Au-thiol bonding and affords a gelatinase-responsive antibacterial photodynamic nanocomposite (GRAPN). In vitro results show that the gelatinase secreted by S. aureus can cleave and release APP from AuNc, thereby resulting in preferential killing of S. aureus over E. coli. In a mouse model of staphylococcal skin wound infection, by integrating gelatinase-responsive drug release and the synergistic effect of a photodynamic agent and APP, GRAPN exhibits a marked photodynamic antibacterial activity, effectively eradicates S. aureus infection, and promotes rapid healing of the infected wounds.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据