Feasible study on poly(Pyrrole-co-Pyrrole-3-Carboxylic Acid)-modified electrode for detection of 17β-Estradiol

Preparation and characterizations of poly(pyrrole-co-pyrrole-3-carboxylic acid) (PPYPA) film on the surface of screen printed carbon electrode (SPE) has been discussed in the manuscript. Different ratios of monomer for PPYPA preparation will be applied to determine 17 beta-estradiol using cyclic voltammetry. The SPE was modified with PPYPA film using an electrodeposition method and followed by the immobilization of 75mer aptamer specifically binding to 17 beta-estradiol as a model study. The materials were characterized by cyclic voltammetry, Fourier transform infrared, electrochemical impedance spectroscopy and scanning electron microscopy. The redox peak potential was found to be at 0.13 V and 0.26 V. The appearance of cauliflower structure in the SEM image which indicates PPYPA film was formed on SPE. Moreover, PPYPA/SPE exhibited a lower charge transfer resistance (Rct) compared to bare SPE indicates a good conductivity of film. Our study shows that 3:1 ratio between PPY and pyrrole-3-carboxylic acid provided highly active surface area for the immobilization of 75mer aptamer for 17 beta-estradiol detection.

Automated summary

Preparation and characterization of poly(pyrrole-co-pyrrole-3-carboxylic acid) film on screen printed carbon electrode (SPE) were discussed in the manuscript. Different ratios of monomer were used for PPYPA preparation for 17 beta-estradiol detection using cyclic voltammetry, showing a good conductivity of the film.