Effect of Biotin on Cardiac Troponin I and High Sensitivity Cardiac Troponin I Assays on Vista 1500 and ADVIA Centaur Analyzer

Objective. Biotin interferes with biotinylated antibody based immunoassays. We investigated effect of biotin on conventional troponin I and two high sensitivity troponin I assays, all manufactured by Siemens. Materials and Methods. One high sensitivity troponin I assay (TNIH-1) was run using ADVIA Centaur analyzer. The second high sensitivity troponin I assay (TNIH-2) as well as conventional troponin I assay (CTNI) were run using Dimension Vista 1500 analyzer. We analyzed 25 specimens using CTNI, TNIH-1 and TNIH-2 assays for comparison of these assays. Moreover, serum pools prepared from additional specimens containing various amounts of troponin I were further supplemented with biotin to achieve biotin concentrations between 50 and 1000 ng/mL followed by reanalysis using CTNI, TNIH-1 and TNIH-2 assays. Results. Although both high sensitivity troponin I assays correlated well, there was a significant positive bias with TNIH-2. We observed no significant negative biotin interference at a level up to 250 ng/mL. Highest observed negative bias was 29.7%. Conclusions. All three troponin I assays were free from biotin interferences up to a biotin concentration of 250 ng/mL.

Automated summary

This study investigated the effects of biotin on troponin I assays and found that all three assays were free from biotin interferences up to a biotin concentration of 250 ng/mL. However, a significant positive bias was observed with one of the high sensitivity troponin I assays.