期刊
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
卷 20, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/1533033821997829
关键词
long noncoding RNA; LBX2 antisense RNA 1; colorectal cancer; prognosis; therapy
类别
资金
- Science and Technology Funding Project of Hunan Province, China [2017SK4010]
- Key laboratory of tumor precision medicine, Hunan colleges and university project [2019-379]
LBX2-AS1 is overexpressed in CRC cell lines, associated with poor prognosis in patients, and knockdown can attenuate the proliferative ability of CRC cells. The study suggests LBX2-AS1 plays a crucial role in the pathogenesis of CRC, providing a potential therapeutic target.
Background: LBX2 antisense RNA 1 (LBX2-AS1), a long noncoding RNA, has been identified to be closely associated with the progression of various cancers. However, the role of LBX2-AS1 in colorectal cancer (CRC) is still poorly understood. In this study, we aimed to investigate the expression and function of LBX2-AS1 in CRC. Material and Methods: Expression data from the Gene Expression Omnibus (GEO) and Gene Expression Profiling Interactive Analysis (GEPIA) databases and results obtained from clinical samples/patients were used to determine the correlation between LBX2-AS1 expression and pathological stages, overall survival (OS). Furthermore, knockdown of LBX2-AS1 in CRC cells using the short interfering RNA (siRNA) technique, and observed its biological functions using western blotting, quantitative reverse transcription-polymerase chain reaction (qRT-PCR), cell counting kit-8 (CCK-8) and flow cytometry assay in the CRC cell line. Results: Our study demonstrated that the expression levels of LBX2-AS1 were higher in CRC cell lines than in normal colon mucosal cell lines. Bioinformatics analysis revealed that CRC patients with high LBX2-AS1 expression levels had poor OS. Furthermore, knockdown of LBX2-AS1 in CRC cells could attenuate the proliferative ability of CRC cells in vitro, which is associated with decreased expression of cyclin-dependent kinase (CDK) 3, CDK6, and CCND1 and enhanced expression of cyclin-dependent kinase inhibitor 1A. Conclusions: LBX2-AS1 plays a crucial role in the tumorigenesis of CRC, providing a potential therapeutic target for CRC patients.
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