期刊
JOURNAL OF PROTEOME RESEARCH
卷 20, 期 2, 页码 1217-1228出版社
AMER CHEMICAL SOC
DOI: 10.1021/acs.jproteome.0c00596
关键词
liquid chromatography; mass spectrometry; peptidomics; genomics; neuropeptides; tissue mapping
资金
- National Science Foundation of China [31801968, 31702021, 31900355]
- Department of Science and Technology in Guangdong Province [2019B090905003, 2019A0102006]
- Shenzhen Science and Technology Program [KQTD20180411143628272]
Through an integrated approach of genomics and peptidomics, this study identified 35 conserved neuropeptides and a potential novel neuropeptide in the American cockroach. The research provides a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, laying a solid foundation for future functional studies of neuropeptides in this insect species.
As a model hemimetabolous insect species and an invasive urban pest that is globally distributed, the American cockroach, Periplaneta americana, is of great interest in both basic and applied research. Previous studies on P. americana neuropeptide identification have been based on biochemical isolation and molecular cloning. In the present study, an integrated approach of genomics- and peptidomics-based discovery was performed for neuropeptide identification in this insect species. First, 67 conserved neuropeptide or neurohormone precursor genes were predicted via an in silico analysis of the P. americana genome and transcriptome. Using a large-scale peptidomic analysis of peptide extracts from four different tissues (the central nervous system, corpora cardiac and corpora allata complex, midgut, and male accessory gland), 35 conserved (predicted) neuropeptides and a potential (novel) neuropeptide were then identified. Subsequent experiments revealed the tissue distribution, sex difference, and developmental patterns of two conserved neuropeptides (allatostatin B and short neuropeptide F) and a novel neuropeptide (PaOGS36577). Our study shows a comprehensive neuropeptidome and detailed spatiotemporal distribution patterns, providing a solid basis for future functional studies of neuropeptides in the American cockroach (data are available via ProteomeXchange with identifier PXD021660).
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