Progress on Cell-free Protein Synthesis Inside Artificial Cells

Cell-free protein synthesis is an in-vitro cell-free expression method for protein synthesis. High-throughput protein expression and in-vitro reconstitution of membrane proteins can be realized in CFPS-containing artificial cells. This review describes various CFPS systems including E. coli extracts, rabbit reticulocytes extracts, wheat germ extracts, and yeast extracts, and summarizes the progress of cell free protein synthesis inside artificial cells. The challenges and the future directions of this field are also proposed at the end of this paper.

Automated summary

Cell-free protein synthesis is an in-vitro method for expressing proteins, allowing high-throughput protein expression and reconstitution of membrane proteins in artificial cells. This review discusses various CFPS systems and the progress of cell-free protein synthesis inside artificial cells, as well as proposing challenges and future directions in the field.