PRODUCTION OF TRIPLOID, DOUBLED HAPLOID (DH) AND MEIOGYNOGENETIC BROOK TROUT (SALVELINUS FONTINALIS)- EFFICIENCY AND DEVELOPMENT OF BODY DEFORMITIES

In the present research we produced triploid, mitogynogenetic (doubled haploid; DH) and meiogynogenetic brook trout (Si:tedious fontistalis) to examine efficiency of these technologies and potential susceptibility of chromosome set manipulated individuals for the spinal disorders. Triploidy was induced by shocking (High Hydrostatic Pressure - HIP) of fertilized eggs 30 min. after insemination. In turn, gvnogenetic development was induced by activation of eggs with UV-irradiated sperm. Activated eggs were then exposed to HHP shock applied 30 and 420 minutes after insemination to provide meiogynogenotes and gynogenetic DlIs, respectively. When compared to non-manipulated diploids, the highest survival rates were observed among triploid brook trout while DHs showed the highest mortality. Malformation rates in the diploid larvae from the control groups did not exceed 7.0% while percentage of malformed triploid individuals equaled 19.1%. Drastically increased number of deformed larvae (> 30%) was observed in both DH and meioanogenetic individuals. Intensification of kyphosis and scoliosis was clearly demonstrated in the gynogenetic and triploid brook trout. Genetic factors such as increased number of sets of chromosomes in triploids and expression of lethal alleles in the gynogenetic fish plus side effects of HHP shock utilized for retention of the second polar body or inhibition of the first cell cleavage when induced triploid and gynogenetic development have been discussed to affect survival rates and prevalence for the skeletal deformities in the chromosome set manipulated brook trout.

Automated summary

In this study, triploid, mitogynogenetic (doubled haploid; DH) and meiogynogenetic brook trout were produced to assess the efficiency of these technologies and the potential susceptibility of chromosome set manipulated individuals for spinal disorders. The highest survival rates were observed among triploid brook trout, while DHs showed the highest mortality. Additionally, significantly increased malformation rates were observed in meiogynogenetic and mitogynogenetic individuals compared to non-manipulated diploids. The study discusses genetic factors and side effects of HHP shock that may impact survival rates and prevalence of skeletal deformities in chromosome set manipulated brook trout.