4.7 Review

Limitations of oxygen delivery to cells in culture: An underappreciated problem in basic and translational research

期刊

FREE RADICAL BIOLOGY AND MEDICINE
卷 113, 期 -, 页码 311-322

出版社

ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2017.10.003

关键词

Hypoxia; Anoxia; Hyperoxia; Hypoxia-inducible factor; HIF; Prolyl-hydroxylase; Oxygen; Diffusion constant; Diffusion gradients; Nitrogen; Carbon dioxide; Gasses; Cell culture; Cell lines; Reactive oxygen species; Mitochondria; Respiration; Glycolysis; Oxidative phosphorylation; Metabolism

资金

  1. National Heart, Lung, and Blood Institute (NHLBI) [R00HL123471]
  2. National Cancer Institute (NCI) [P30CA086862]

向作者/读者索取更多资源

Molecular oxygen is one of the most important variables in modern cell culture systems. Fluctuations in its concentration can affect cell growth, differentiation, signaling, and free radical production. In order to maintain culture viability, experimental validity, and reproducibility, it is imperative that oxygen levels be consistently maintained within physiological normoxic limits. Use of the term normoxia, however, is not consistent among scientists who experiment in cell culture. It is typically used to describe the atmospheric conditions of a standard incubator, not the true microenvironment to which the cells are exposed. This error may lead to the situation where cells grown in a standard normoxic oxygen concentration may actually be experiencing a wide range of conditions ranging from hyperoxia to near-anoxic conditions at the cellular level. This apparent paradox is created by oxygen's sluggish rate of diffusion through aqueous medium, and the generally underappreciated effects that cell density, media volume, and barometric pressure can have on pericellular oxygen concentration in a cell culture system. This review aims to provide an overview of this phenomenon we have termed consumptive oxygen depletion (COD), and includes a basic review of the physics, potential consequences, and alternative culture methods currently available to help circumvent this largely unrecognized problem.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据