期刊
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
卷 8, 期 4, 页码 -出版社
MDPI
DOI: 10.3390/jcdd8040041
关键词
heart development; first heart field; lineage tracing; cardiac ventricle; zebrafish
资金
- Department of Pediatrics, University of Colorado School of Medicine Anschutz Medical Campus
- Children's Hospital Colorado Foundation
- Swiss National Science Foundation
- Novartis Foundation for medical-biological research
The study reveals the presence of myocardial cells derived from FHF in both developing and adult zebrafish hearts, predominantly located in the outer curvature and right side of the ventricle in embryonic stages. These lineage-labeled cells persist in adulthood, positioned in a region opposite to the atrium and encompassing the apex, indicating a cell-based compartmentalization of the adult zebrafish ventricle.
The vertebrate heart integrates cells from the early-differentiating first heart field (FHF) and the later-differentiating second heart field (SHF), both emerging from the lateral plate mesoderm. In mammals, this process forms the basis for the development of the left and right ventricle chambers and subsequent chamber septation. The single ventricle-forming zebrafish heart also integrates FHF and SHF lineages during embryogenesis, yet the contributions of these two myocardial lineages to the adult zebrafish heart remain incompletely understood. Here, we characterize the myocardial labeling of FHF descendants in both the developing and adult zebrafish ventricle. Expanding previous findings, late gastrulation-stage labeling using drl-driven CreERT2 recombinase with a myocardium-specific, myl7-controlled, loxP reporter results in the predominant labeling of FHF-derived outer curvature and the right side of the embryonic ventricle. Raised to adulthood, such lineage-labeled hearts retain broad areas of FHF cardiomyocytes in a region of the ventricle that is positioned at the opposite side to the atrium and encompasses the apex. Our data add to the increasing evidence for a persisting cell-based compartmentalization of the adult zebrafish ventricle even in the absence of any physical boundary.
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