期刊
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH
卷 54, 期 5, 页码 -出版社
ASSOC BRAS DIVULG CIENTIFICA
DOI: 10.1590/1414-431X202010185
关键词
Intervertebral disc degeneration; Nucleus pulposus cells; mTORC1; Lactic acid; Apoptosis
This study revealed a novel mechanism by which mTORC1 signaling controls the survival of nucleus pulposus cells, offering potential strategies for clinical intervention in lumbar disc herniation. By inhibiting mTORC1 activity, nucleus pulposus cell survival can be promoted, showing the importance of metabolic homeostasis in IVD degeneration.
Lumbar disc herniation is a common disease characterized by the degeneration of intervertebral discs (IVDs), accompanied by imbalance of metabolic and inflammatory homeostasis. Current studies establish that IVD degeneration is induced by increased apoptosis of nucleus pulposus (NP) cells. However, the underlying mechanisms of NP cell survival/apoptosis are not well elucidated. Here, we reveal a novel mechanism by which mTORC1 signaling controls NP cell survival through regulating metabolic homeostasis. We demonstrated that hyperactivated mTORC1 activity induced by inflammatory cytokines engenders the apoptosis of NP cells, whereas pharmacological inhibition of mTORC1 activity promotes NP cell survival. Using an integrative approach spanning metabolomics and biochemical approaches, we showed that mTORC1 activation enhanced glucose metabolism and lactic acid production, and therefore caused NP cell apoptosis. Our study identified mTORC1 in NP cells as a novel target for IVD degeneration, and provided potential strategies for clinical intervention of lumbar disc herniation.
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