Metal-responsive reversible binding of triplex-forming oligonucleotides with 5-hydroxyuracil nucleobases

Metal-responsive triplex-forming oligonucleotides (TFOs) were synthesised by incorporating 5-hydroxyuracil (UOH) nucleobases as metal recognition sites. Binding of the UOH-containing TFO to the target natural DNA duplexes was reversibly regulated by the addition and removal of Gd-III ions under isothermal conditions.

Automated summary

Metal-responsive triplex-forming oligonucleotides incorporating 5-hydroxyuracil nucleobases were synthesized, showing reversible regulation of binding to target natural DNA duplexes by the addition and removal of Gd-III ions under isothermal conditions.