期刊
LAB ON A CHIP
卷 21, 期 5, 页码 867-874出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc01032d
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- Human Resources Program in Energy Technology of the Korea Institute of Energy Technology Evaluation and Planning (KETEP) from the Ministry of Trade, Industry & Energy, Republic of Korea [20194010201830]
- Ministry of SMEs and Startups (MSS, Korea) [S2848425]
- Korea Evaluation Institute of Industrial Technology (KEIT) [20194010201830] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
- Korea Technology & Information Promotion Agency for SMEs (TIPA) [S2848425] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
Ion concentration polarization (ICP) is a preconcentration technique with the potential for high preconcentration factor, but faces challenges of instability and low efficiency under physiological conditions. A sequentially driven ICP process has been proposed to enhance the preconcentration of highly ionic raw samples without increasing the electric field, resulting in significant preconcentration factor and signal enhancement in enzyme-linked immunosorbent assay (ELISA) using tau protein.
Ion concentration polarization (ICP) is one of the preconcentration techniques which can acquire a high preconcentration factor. Still, the main hurdles of ICP are its instability and low efficiency under physiological conditions with high ionic strength and abundant biomolecules. Here, we suggested a sequentially driven ICP process, which enhanced the electrokinetic force required for preconcentration, enabling enrichment of highly ionic raw samples without increasing the electric field. We acquired a 13-fold preconcentration factor (PF) in human serum using a paper-based origami structure consisting of multiple layers for three-dimensional sequential ICP (3D seq-ICP). Moreover, we demonstrated a paper-based enzyme-linked immunosorbent assay (ELISA) by 3D seq-ICP using tau protein, showing a 6-fold increase in ELISA signals.
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