期刊
FORENSIC SCIENCE INTERNATIONAL-GENETICS
卷 29, 期 -, 页码 29-37出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigen.2017.03.014
关键词
Microhaplotype; SNP; Ancestry; Massively parallel sequencing
资金
- NIJ by the National Institute of Justice, Office of Justice Programs, U.S. Department of Justice [2013-DN-BX-K023, 2014-DN-BX-K030]
Today the primary DNA markers used in forensics are short tandem repeat (STR) polymorphisms (STRPs), initially selected because they are highly polymorphic. However, the increasingly common need to deal with samples with a mixture of DNA from two or more individuals sometimes is complicated by the inherent stutter involved with PCR amplification, especially in strongly unbalanced mixtures when the minor component coincides with the stutter range of the major component. Also, the STRPs in use provide little evidence of ancestry of a single source sample beyond broad continental resolution. Methodologies for analyzing DNA have become much more powerful in recent years. Massively parallel sequencing (MPS) is a new method being considered for routine use in forensics. Primarily to aid in mixture deconvolution and avoid the issue of stutter, we have begun to investigate a new type of forensic marker, microhaplotype loci, that will provide useful information on mixtures of DNA and on ancestry when typed using massively parallel sequencing (MPS). We have identified 130 loci and estimated their haplotype (allele) frequencies in 83 different population samples. Many of these loci are shown to be highly informative for individual identification and for mixture identification and deconvolution. (C) 2017 The Authors. Published by Elsevier Ireland Ltd. This is an open access article under the CC BY-NCND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
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