期刊
FORENSIC SCIENCE INTERNATIONAL-GENETICS
卷 29, 期 -, 页码 174-180出版社
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigen.2017.04.003
关键词
Mitochondrial DNA; Next-generation sequencing (NGS); KAPA HyperPlus; Nextera XT
资金
- Rapid Reaction Technology Office, ASD (RE)
Next-generation sequencing (NGS) facilitates the rapid and high-throughput generation of human mitochondrial genome (mitogenome) data to build population and reference databases for forensic comparisons. To this end, long-range amplification provides an effective method of target enrichment that is amenable to library preparation assays employing DNA fragmentation. This study compared the Nextera XT DNA Library Preparation Kit (Illumina, San Diego, CA) and the KAPA HyperPlus Library Preparation Kit (Kapa Biosystems, Wilmington, MA) for enzymatic fragmentation and indexing of similar to 8500 bp mitogenome amplicons for Illumina sequencing. The Nextera XT libraries produced low-coverage regions that were consistent across all samples, while the HyperPlus libraries resulted in uniformly high coverage across the mitogenome, even with reduced-volume reaction conditions. The balanced coverage observed from KAPA HyperPlus libraries enables not only low-level variant calling across the mitogenome but also increased sample multiplexing for greater processing efficiency. (C) 2017 Elsevier B.V. All rights reserved.
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