Vessel-on-a-chip models for studying microvascular physiology, transport, and function in vitro

To understand how the microvasculature grows and remodels, researchers require reproducible systems that emulate the function of living tissue. Innovative contributions toward fulfilling this important need have been made by engineered microvessels assembled in vitro with microfabrication techniques. Microfabricated vessels, commonly referred to as vessels-on-a-chip, are from a class of cell culture technologies that uniquely integrate microscale flow phenomena, tissue-level biomolecular transport, cell-cell interactions, and proper three-dimensional (3-D) extracellular matrix environments under well-defined culture conditions. Here, we discuss the enabling attributes of microfabricated vessels that make these models more physiological compared with established cell culture techniques and the potential of these models for advancing microvascular research. This review highlights the key features of microvascular transport and physiology, critically discusses the strengths and limitations of different microfabrication strategies for studying the microvasculature, and provides a perspective on current challenges and future opportunities for vessel-on-a-chip models.

Automated summary

Microfabricated vessels, also known as vessels-on-a-chip, integrate microscale flow phenomena, tissue-level biomolecular transport, cell-cell interactions, and 3-D extracellular matrix environments. These models offer more physiological features compared with traditional cell culture techniques and have great potential for advancing microvascular research.