期刊
ACS APPLIED BIO MATERIALS
卷 4, 期 3, 页码 2135-2141出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.0c01546
关键词
pH probe; near-infrared emission; ratiometric imaging; stomach cancer; two-photon microscopy; tissue imaging
资金
- National Leading Research Lab Program of the National Research Foundation of Korea (NRF) - Korean government (MSIP) [NRF-2019R1A2B5B03100278]
- Center for Convergence Research of Neurological Disorders [NRF-2019R1A5A2026045]
- GRRC program of Gyeonggi province [GRRCAJOU2019B02]
- Ajou University research fund
Stomach cancer is a global health issue with high incidence and mortality rates. The development of the HCC1 probe allows for quantitative analysis of pH in live cells and human stomach tissues, showing promising results for in situ pH-related studies and cancer research.
Stomach cancer is a global health issue because of its incidence and mortality rates worldwide. We developed a near-infrared (NIR) emissive ratiometric two-photon (TP) probe (HCC1) for the quantitative analysis of pH in live cells and human stomach tissues. The probe design is based on a restrained hemicyanine core that controls the intramolecular charge transfer from 2- naphthol, with a suitable pK(a) value (7.50) under physiological conditions. The probe exhibited improved quantum yield, stability, and TP activity under physiological conditions. In addition, intracellular pH titration (pH 4.0 to 10.0) of HCC1 revealed an ideal intracellular pK(a) of approximately 7.2, negligible cytotoxicity, and TP excited fluorescence in situ, thereby allowing direct imaging of the cellular pH in live cells and tissues. Ratiometric two-photon microscope imaging with HCC1 of human stomach tissue revealed a clear intratissue pH variation among normal, adenoma, and cancer tissues. Our results demonstrate that HCC1 is useful as an NIR imaging probe for in situ pH-related studies and in cancer research.
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